Identification of phosphohistidine in digests from a probable intermediate of oxidative phosphorylation.
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چکیده
منابع مشابه
Relationships of Ca++ to "protein-bound" Phosphate Fractions of Mitochondria.
The active transport of K+, Mg++, Mn++, and Ca++ ions by mitochondria appears to be int,imately related in some manner to the process of oxidative phosphorylation (l-7). As suggested by Vasington and Murphy (6), and further amplified by Brierley, Murer, and Green (S), ion transport may utilize “high energy” intermediates produced by mitochondrial oxidative phosphorylation. Thus energy for ion t...
متن کاملIdentification and characterization of a mammalian 14-kDa phosphohistidine phosphatase.
Protein histidine phosphorylation in eukaryotes has been sparsely studied compared to protein serine/threonine and tyrosine phosphorylation. In an attempt to rectify this by probing porcine liver cytosol with the phosphohistidine-containing peptide succinyl-Ala-His(P)-Pro-Phe-p-nitroanilide (phosphopeptide I), we observed a phosphatase activity that was insensitive towards okadaic acid and EDTA...
متن کاملMonoclonal 1- and 3-Phosphohistidine Antibodies: New Tools to Study Histidine Phosphorylation
Histidine phosphorylation (pHis) is well studied in bacteria; however, its role in mammalian signaling remains largely unexplored due to the lack of pHis-specific antibodies and the lability of the phosphoramidate (P-N) bond. Both imidazole nitrogens can be phosphorylated, forming 1-phosphohistidine (1-pHis) or 3-phosphohistidine (3-pHis). We have developed monoclonal antibodies (mAbs) that spe...
متن کاملImmunohistochemical localization of phosphohistidine phosphatase PHPT1 in mouse and human tissues
Protein histidine phosphorylation accounts for about 6% of the total protein phosphorylation in eukaryotic cells; still details concerning histidine phosphorylation and dephosphorylation are limited. A mammalian 14-kDa phosphohistidine phosphatase, also denominated PHPT1, was found 6 years ago that provided a new tool in the study of phosphohistidine phosphorylation. The localization of PHPT1 m...
متن کاملHistidine phosphorylation of P-selectin upon stimulation of human platelets: A novel pathway for activation-dependent signal transduction
Transient phosphorylation of histidine characterizes the two-component systems in prokaryotes that control important physiological functions, but analogous events have not been implicated in signal transduction in mammalian cells. To explore histidine phosphorylation during activation of human cells, stimulated platelets were analyzed for the formation of protein phosphohistidine in a model sys...
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ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 237 شماره
صفحات -
تاریخ انتشار 1962